Journal: JOR Spine

Article Title: Age‐related molecular changes in the lumbar dorsal root ganglia of mice: Signs of sensitization, and inflammatory response

doi: 10.1002/jsp2.1124

Figure Lengend Snippet: List of primary and secondary antibodies used for immunofluorescence analysis

Article Snippet: rabbit anti‐NGF , BosterBio , m00341 , 1.00 , 1:100 , same as above.

Techniques: Immunofluorescence, Concentration Assay

Journal: Molecular imaging and biology

Article Title: PET detection of cerebral necrosis using an infarct-avid agent 2-deoxy-2-[ 18 F]fluoro-D-glucaric acid (FGA) in a mouse model of the brain stroke

doi: 10.1007/s11307-020-01513-9

Figure Lengend Snippet: Concentration of neurotrophin levels in the brain. (A) BDNF levels (n=5). (B) NGF-β levels (n = 5 from stroke group and n = 6 from sham group).

Article Snippet: Brain-derived nerve factor (BDNF) and nerve growth factor (NGF)-β Mouse BDNF and NGF-β ELISA kits were purchased from Boster Biochem (Pleasanton, CA).

Techniques: Concentration Assay

Journal: NPJ Regenerative Medicine

Article Title: Ultrasound-guided platelet-rich plasma injection and multimodality ultrasound examination of peripheral nerve crush injury

doi: 10.1038/s41536-020-00101-3

Figure Lengend Snippet: a Immunostaining of harvested SCs. b SCs exposed to various PRP concentrations during 5 days of culture. c , d displayed the SCs count during 3 days and 5 days of culture (* P < 0.05, ** P < 0.01). e CCK-8 colorimetric assay was performed to evaluate the effect of PRP in various concentration on the proliferation of SCs (** P < 0.01 vs. control group; †† P < 0.01 vs. PRP-6.5× group; ‡‡ P < 0.05 vs. other five groups). f The NGF-β, VEGF and GDNF secreted by SCs that cultured with PRP at different concentrations were measured by ELISA at 5 days of culture (* P < 0.05 ** P < 0.01 vs. control group; †† P < 0.01 vs. PRP-6.5× group; ‡‡ P < 0.05 vs. other five groups). Error bars = SD, n = 3. SCs Schwann cells, PRP platelet-rich plasma, CCK-8 Cell Counting Kit-8.

Article Snippet: After 5 days of treatment with the indicated PRP, the culture medium was collected, and the concentrations of NGF-β, VEGF, and GDNF in the culture medium were determined using an NGF-β ELISA kit (Boster, EK0471), a VEGF ELISA kit (Boster, EK0540) and a GDNF ELISA kit (EK0363) as instructed by the manufacturer.

Techniques: Immunostaining, CCK-8 Assay, Colorimetric Assay, Concentration Assay, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Cell Counting